guard column Search Results


brownlee spp hplc column  (Revvity)
91
Revvity brownlee spp hplc column
Changes in prostatic acid phosphatase (PAP) ectonucleotidase activity and alterations of adenosine hydrolysis in resiniferatoxin (RTX) neuropathy. (A–C) Prostatic acid phosphatase ectonucleotidase activity was assessed through enzyme histochemistry using adenosine monophosphate (AMP, 3 mM) as a substrate. The reaction product was developed with 1% sodium sulfide and appeared dark brown in the dorsal root ganglion (DRG) sections of the vehicle (A) and day 7 after RTX neuropathy (RTX group) (B). (C) The graph shows AMP(+) neuronal densities in the vehicle (open bar, n = 5) and RTX (filled bar, n = 5) groups according to (A and B). (D–G) High-performance liquid chromatography <t>(HPLC)</t> was conducted to assess the change in the contents of adenosine and AMP, which were extracted from DRG. (D) This graph shows the HPLC chromatograms of the eluted standards (upper panel; AMP, 6.5 minutes; adenosine, 10.2 minutes), the vehicle group (middle panel), and the RTX group (bottom panel). Number designation on the chromatograms: 1 for AMP and 2 for adenosine. (E and F) These figures provide verification of the peaks for AMP (E) and adenosine (F) samples extracted from DRG tissues with respect to a spectral library. The absorbance spectra of the adenosine and AMP samples (pink line in E and F) were the same as the absorbance spectra of the adenosine standard (similarity index = 0.99) and the AMP standard (similarity index = 1.00) (black line in E and F). (G) A significant difference in adenosine signaling molecules was present between the vehicle (open bar, n = 6) and RTX groups (filled bar, n = 5) (ie, decreased adenosine and increased AMP). Elution time scale, minutes. * P < 0.05, ** P < 0.01. Bar, 50 µm.
Brownlee Spp Hplc Column, supplied by Revvity, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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preparative hplc  (MACHEREY NAGEL)
93
MACHEREY NAGEL preparative hplc
Changes in prostatic acid phosphatase (PAP) ectonucleotidase activity and alterations of adenosine hydrolysis in resiniferatoxin (RTX) neuropathy. (A–C) Prostatic acid phosphatase ectonucleotidase activity was assessed through enzyme histochemistry using adenosine monophosphate (AMP, 3 mM) as a substrate. The reaction product was developed with 1% sodium sulfide and appeared dark brown in the dorsal root ganglion (DRG) sections of the vehicle (A) and day 7 after RTX neuropathy (RTX group) (B). (C) The graph shows AMP(+) neuronal densities in the vehicle (open bar, n = 5) and RTX (filled bar, n = 5) groups according to (A and B). (D–G) High-performance liquid chromatography <t>(HPLC)</t> was conducted to assess the change in the contents of adenosine and AMP, which were extracted from DRG. (D) This graph shows the HPLC chromatograms of the eluted standards (upper panel; AMP, 6.5 minutes; adenosine, 10.2 minutes), the vehicle group (middle panel), and the RTX group (bottom panel). Number designation on the chromatograms: 1 for AMP and 2 for adenosine. (E and F) These figures provide verification of the peaks for AMP (E) and adenosine (F) samples extracted from DRG tissues with respect to a spectral library. The absorbance spectra of the adenosine and AMP samples (pink line in E and F) were the same as the absorbance spectra of the adenosine standard (similarity index = 0.99) and the AMP standard (similarity index = 1.00) (black line in E and F). (G) A significant difference in adenosine signaling molecules was present between the vehicle (open bar, n = 6) and RTX groups (filled bar, n = 5) (ie, decreased adenosine and increased AMP). Elution time scale, minutes. * P < 0.05, ** P < 0.01. Bar, 50 µm.
Preparative Hplc, supplied by MACHEREY NAGEL, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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nucleodur c 18 gravity column  (MACHEREY NAGEL)
93
MACHEREY NAGEL nucleodur c 18 gravity column
Changes in prostatic acid phosphatase (PAP) ectonucleotidase activity and alterations of adenosine hydrolysis in resiniferatoxin (RTX) neuropathy. (A–C) Prostatic acid phosphatase ectonucleotidase activity was assessed through enzyme histochemistry using adenosine monophosphate (AMP, 3 mM) as a substrate. The reaction product was developed with 1% sodium sulfide and appeared dark brown in the dorsal root ganglion (DRG) sections of the vehicle (A) and day 7 after RTX neuropathy (RTX group) (B). (C) The graph shows AMP(+) neuronal densities in the vehicle (open bar, n = 5) and RTX (filled bar, n = 5) groups according to (A and B). (D–G) High-performance liquid chromatography <t>(HPLC)</t> was conducted to assess the change in the contents of adenosine and AMP, which were extracted from DRG. (D) This graph shows the HPLC chromatograms of the eluted standards (upper panel; AMP, 6.5 minutes; adenosine, 10.2 minutes), the vehicle group (middle panel), and the RTX group (bottom panel). Number designation on the chromatograms: 1 for AMP and 2 for adenosine. (E and F) These figures provide verification of the peaks for AMP (E) and adenosine (F) samples extracted from DRG tissues with respect to a spectral library. The absorbance spectra of the adenosine and AMP samples (pink line in E and F) were the same as the absorbance spectra of the adenosine standard (similarity index = 0.99) and the AMP standard (similarity index = 1.00) (black line in E and F). (G) A significant difference in adenosine signaling molecules was present between the vehicle (open bar, n = 6) and RTX groups (filled bar, n = 5) (ie, decreased adenosine and increased AMP). Elution time scale, minutes. * P < 0.05, ** P < 0.01. Bar, 50 µm.
Nucleodur C 18 Gravity Column, supplied by MACHEREY NAGEL, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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c18 nucleosil  (MACHEREY NAGEL)
92
MACHEREY NAGEL c18 nucleosil
Changes in prostatic acid phosphatase (PAP) ectonucleotidase activity and alterations of adenosine hydrolysis in resiniferatoxin (RTX) neuropathy. (A–C) Prostatic acid phosphatase ectonucleotidase activity was assessed through enzyme histochemistry using adenosine monophosphate (AMP, 3 mM) as a substrate. The reaction product was developed with 1% sodium sulfide and appeared dark brown in the dorsal root ganglion (DRG) sections of the vehicle (A) and day 7 after RTX neuropathy (RTX group) (B). (C) The graph shows AMP(+) neuronal densities in the vehicle (open bar, n = 5) and RTX (filled bar, n = 5) groups according to (A and B). (D–G) High-performance liquid chromatography <t>(HPLC)</t> was conducted to assess the change in the contents of adenosine and AMP, which were extracted from DRG. (D) This graph shows the HPLC chromatograms of the eluted standards (upper panel; AMP, 6.5 minutes; adenosine, 10.2 minutes), the vehicle group (middle panel), and the RTX group (bottom panel). Number designation on the chromatograms: 1 for AMP and 2 for adenosine. (E and F) These figures provide verification of the peaks for AMP (E) and adenosine (F) samples extracted from DRG tissues with respect to a spectral library. The absorbance spectra of the adenosine and AMP samples (pink line in E and F) were the same as the absorbance spectra of the adenosine standard (similarity index = 0.99) and the AMP standard (similarity index = 1.00) (black line in E and F). (G) A significant difference in adenosine signaling molecules was present between the vehicle (open bar, n = 6) and RTX groups (filled bar, n = 5) (ie, decreased adenosine and increased AMP). Elution time scale, minutes. * P < 0.05, ** P < 0.01. Bar, 50 µm.
C18 Nucleosil, supplied by MACHEREY NAGEL, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rp hplc analytical runs  (MACHEREY NAGEL)
94
MACHEREY NAGEL rp hplc analytical runs
Changes in prostatic acid phosphatase (PAP) ectonucleotidase activity and alterations of adenosine hydrolysis in resiniferatoxin (RTX) neuropathy. (A–C) Prostatic acid phosphatase ectonucleotidase activity was assessed through enzyme histochemistry using adenosine monophosphate (AMP, 3 mM) as a substrate. The reaction product was developed with 1% sodium sulfide and appeared dark brown in the dorsal root ganglion (DRG) sections of the vehicle (A) and day 7 after RTX neuropathy (RTX group) (B). (C) The graph shows AMP(+) neuronal densities in the vehicle (open bar, n = 5) and RTX (filled bar, n = 5) groups according to (A and B). (D–G) High-performance liquid chromatography <t>(HPLC)</t> was conducted to assess the change in the contents of adenosine and AMP, which were extracted from DRG. (D) This graph shows the HPLC chromatograms of the eluted standards (upper panel; AMP, 6.5 minutes; adenosine, 10.2 minutes), the vehicle group (middle panel), and the RTX group (bottom panel). Number designation on the chromatograms: 1 for AMP and 2 for adenosine. (E and F) These figures provide verification of the peaks for AMP (E) and adenosine (F) samples extracted from DRG tissues with respect to a spectral library. The absorbance spectra of the adenosine and AMP samples (pink line in E and F) were the same as the absorbance spectra of the adenosine standard (similarity index = 0.99) and the AMP standard (similarity index = 1.00) (black line in E and F). (G) A significant difference in adenosine signaling molecules was present between the vehicle (open bar, n = 6) and RTX groups (filled bar, n = 5) (ie, decreased adenosine and increased AMP). Elution time scale, minutes. * P < 0.05, ** P < 0.01. Bar, 50 µm.
Rp Hplc Analytical Runs, supplied by MACHEREY NAGEL, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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c 4 nucleosil guard column  (MACHEREY NAGEL)
93
MACHEREY NAGEL c 4 nucleosil guard column
Changes in prostatic acid phosphatase (PAP) ectonucleotidase activity and alterations of adenosine hydrolysis in resiniferatoxin (RTX) neuropathy. (A–C) Prostatic acid phosphatase ectonucleotidase activity was assessed through enzyme histochemistry using adenosine monophosphate (AMP, 3 mM) as a substrate. The reaction product was developed with 1% sodium sulfide and appeared dark brown in the dorsal root ganglion (DRG) sections of the vehicle (A) and day 7 after RTX neuropathy (RTX group) (B). (C) The graph shows AMP(+) neuronal densities in the vehicle (open bar, n = 5) and RTX (filled bar, n = 5) groups according to (A and B). (D–G) High-performance liquid chromatography <t>(HPLC)</t> was conducted to assess the change in the contents of adenosine and AMP, which were extracted from DRG. (D) This graph shows the HPLC chromatograms of the eluted standards (upper panel; AMP, 6.5 minutes; adenosine, 10.2 minutes), the vehicle group (middle panel), and the RTX group (bottom panel). Number designation on the chromatograms: 1 for AMP and 2 for adenosine. (E and F) These figures provide verification of the peaks for AMP (E) and adenosine (F) samples extracted from DRG tissues with respect to a spectral library. The absorbance spectra of the adenosine and AMP samples (pink line in E and F) were the same as the absorbance spectra of the adenosine standard (similarity index = 0.99) and the AMP standard (similarity index = 1.00) (black line in E and F). (G) A significant difference in adenosine signaling molecules was present between the vehicle (open bar, n = 6) and RTX groups (filled bar, n = 5) (ie, decreased adenosine and increased AMP). Elution time scale, minutes. * P < 0.05, ** P < 0.01. Bar, 50 µm.
C 4 Nucleosil Guard Column, supplied by MACHEREY NAGEL, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ec 4 3 nucleoshell rp 18 plus guard column  (MACHEREY NAGEL)
94
MACHEREY NAGEL ec 4 3 nucleoshell rp 18 plus guard column
Changes in prostatic acid phosphatase (PAP) ectonucleotidase activity and alterations of adenosine hydrolysis in resiniferatoxin (RTX) neuropathy. (A–C) Prostatic acid phosphatase ectonucleotidase activity was assessed through enzyme histochemistry using adenosine monophosphate (AMP, 3 mM) as a substrate. The reaction product was developed with 1% sodium sulfide and appeared dark brown in the dorsal root ganglion (DRG) sections of the vehicle (A) and day 7 after RTX neuropathy (RTX group) (B). (C) The graph shows AMP(+) neuronal densities in the vehicle (open bar, n = 5) and RTX (filled bar, n = 5) groups according to (A and B). (D–G) High-performance liquid chromatography <t>(HPLC)</t> was conducted to assess the change in the contents of adenosine and AMP, which were extracted from DRG. (D) This graph shows the HPLC chromatograms of the eluted standards (upper panel; AMP, 6.5 minutes; adenosine, 10.2 minutes), the vehicle group (middle panel), and the RTX group (bottom panel). Number designation on the chromatograms: 1 for AMP and 2 for adenosine. (E and F) These figures provide verification of the peaks for AMP (E) and adenosine (F) samples extracted from DRG tissues with respect to a spectral library. The absorbance spectra of the adenosine and AMP samples (pink line in E and F) were the same as the absorbance spectra of the adenosine standard (similarity index = 0.99) and the AMP standard (similarity index = 1.00) (black line in E and F). (G) A significant difference in adenosine signaling molecules was present between the vehicle (open bar, n = 6) and RTX groups (filled bar, n = 5) (ie, decreased adenosine and increased AMP). Elution time scale, minutes. * P < 0.05, ** P < 0.01. Bar, 50 µm.
Ec 4 3 Nucleoshell Rp 18 Plus Guard Column, supplied by MACHEREY NAGEL, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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nucleodur 100 5 c18 ec  (MACHEREY NAGEL)
93
MACHEREY NAGEL nucleodur 100 5 c18 ec
Changes in prostatic acid phosphatase (PAP) ectonucleotidase activity and alterations of adenosine hydrolysis in resiniferatoxin (RTX) neuropathy. (A–C) Prostatic acid phosphatase ectonucleotidase activity was assessed through enzyme histochemistry using adenosine monophosphate (AMP, 3 mM) as a substrate. The reaction product was developed with 1% sodium sulfide and appeared dark brown in the dorsal root ganglion (DRG) sections of the vehicle (A) and day 7 after RTX neuropathy (RTX group) (B). (C) The graph shows AMP(+) neuronal densities in the vehicle (open bar, n = 5) and RTX (filled bar, n = 5) groups according to (A and B). (D–G) High-performance liquid chromatography <t>(HPLC)</t> was conducted to assess the change in the contents of adenosine and AMP, which were extracted from DRG. (D) This graph shows the HPLC chromatograms of the eluted standards (upper panel; AMP, 6.5 minutes; adenosine, 10.2 minutes), the vehicle group (middle panel), and the RTX group (bottom panel). Number designation on the chromatograms: 1 for AMP and 2 for adenosine. (E and F) These figures provide verification of the peaks for AMP (E) and adenosine (F) samples extracted from DRG tissues with respect to a spectral library. The absorbance spectra of the adenosine and AMP samples (pink line in E and F) were the same as the absorbance spectra of the adenosine standard (similarity index = 0.99) and the AMP standard (similarity index = 1.00) (black line in E and F). (G) A significant difference in adenosine signaling molecules was present between the vehicle (open bar, n = 6) and RTX groups (filled bar, n = 5) (ie, decreased adenosine and increased AMP). Elution time scale, minutes. * P < 0.05, ** P < 0.01. Bar, 50 µm.
Nucleodur 100 5 C18 Ec, supplied by MACHEREY NAGEL, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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nucleodur c18 isis  (MACHEREY NAGEL)
93
MACHEREY NAGEL nucleodur c18 isis
Changes in prostatic acid phosphatase (PAP) ectonucleotidase activity and alterations of adenosine hydrolysis in resiniferatoxin (RTX) neuropathy. (A–C) Prostatic acid phosphatase ectonucleotidase activity was assessed through enzyme histochemistry using adenosine monophosphate (AMP, 3 mM) as a substrate. The reaction product was developed with 1% sodium sulfide and appeared dark brown in the dorsal root ganglion (DRG) sections of the vehicle (A) and day 7 after RTX neuropathy (RTX group) (B). (C) The graph shows AMP(+) neuronal densities in the vehicle (open bar, n = 5) and RTX (filled bar, n = 5) groups according to (A and B). (D–G) High-performance liquid chromatography <t>(HPLC)</t> was conducted to assess the change in the contents of adenosine and AMP, which were extracted from DRG. (D) This graph shows the HPLC chromatograms of the eluted standards (upper panel; AMP, 6.5 minutes; adenosine, 10.2 minutes), the vehicle group (middle panel), and the RTX group (bottom panel). Number designation on the chromatograms: 1 for AMP and 2 for adenosine. (E and F) These figures provide verification of the peaks for AMP (E) and adenosine (F) samples extracted from DRG tissues with respect to a spectral library. The absorbance spectra of the adenosine and AMP samples (pink line in E and F) were the same as the absorbance spectra of the adenosine standard (similarity index = 0.99) and the AMP standard (similarity index = 1.00) (black line in E and F). (G) A significant difference in adenosine signaling molecules was present between the vehicle (open bar, n = 6) and RTX groups (filled bar, n = 5) (ie, decreased adenosine and increased AMP). Elution time scale, minutes. * P < 0.05, ** P < 0.01. Bar, 50 µm.
Nucleodur C18 Isis, supplied by MACHEREY NAGEL, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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nucleodur c18 htec  (MACHEREY NAGEL)
93
MACHEREY NAGEL nucleodur c18 htec
Changes in prostatic acid phosphatase (PAP) ectonucleotidase activity and alterations of adenosine hydrolysis in resiniferatoxin (RTX) neuropathy. (A–C) Prostatic acid phosphatase ectonucleotidase activity was assessed through enzyme histochemistry using adenosine monophosphate (AMP, 3 mM) as a substrate. The reaction product was developed with 1% sodium sulfide and appeared dark brown in the dorsal root ganglion (DRG) sections of the vehicle (A) and day 7 after RTX neuropathy (RTX group) (B). (C) The graph shows AMP(+) neuronal densities in the vehicle (open bar, n = 5) and RTX (filled bar, n = 5) groups according to (A and B). (D–G) High-performance liquid chromatography <t>(HPLC)</t> was conducted to assess the change in the contents of adenosine and AMP, which were extracted from DRG. (D) This graph shows the HPLC chromatograms of the eluted standards (upper panel; AMP, 6.5 minutes; adenosine, 10.2 minutes), the vehicle group (middle panel), and the RTX group (bottom panel). Number designation on the chromatograms: 1 for AMP and 2 for adenosine. (E and F) These figures provide verification of the peaks for AMP (E) and adenosine (F) samples extracted from DRG tissues with respect to a spectral library. The absorbance spectra of the adenosine and AMP samples (pink line in E and F) were the same as the absorbance spectra of the adenosine standard (similarity index = 0.99) and the AMP standard (similarity index = 1.00) (black line in E and F). (G) A significant difference in adenosine signaling molecules was present between the vehicle (open bar, n = 6) and RTX groups (filled bar, n = 5) (ie, decreased adenosine and increased AMP). Elution time scale, minutes. * P < 0.05, ** P < 0.01. Bar, 50 µm.
Nucleodur C18 Htec, supplied by MACHEREY NAGEL, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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nucleodur c18 gravity sb column  (MACHEREY NAGEL)
94
MACHEREY NAGEL nucleodur c18 gravity sb column
Changes in prostatic acid phosphatase (PAP) ectonucleotidase activity and alterations of adenosine hydrolysis in resiniferatoxin (RTX) neuropathy. (A–C) Prostatic acid phosphatase ectonucleotidase activity was assessed through enzyme histochemistry using adenosine monophosphate (AMP, 3 mM) as a substrate. The reaction product was developed with 1% sodium sulfide and appeared dark brown in the dorsal root ganglion (DRG) sections of the vehicle (A) and day 7 after RTX neuropathy (RTX group) (B). (C) The graph shows AMP(+) neuronal densities in the vehicle (open bar, n = 5) and RTX (filled bar, n = 5) groups according to (A and B). (D–G) High-performance liquid chromatography <t>(HPLC)</t> was conducted to assess the change in the contents of adenosine and AMP, which were extracted from DRG. (D) This graph shows the HPLC chromatograms of the eluted standards (upper panel; AMP, 6.5 minutes; adenosine, 10.2 minutes), the vehicle group (middle panel), and the RTX group (bottom panel). Number designation on the chromatograms: 1 for AMP and 2 for adenosine. (E and F) These figures provide verification of the peaks for AMP (E) and adenosine (F) samples extracted from DRG tissues with respect to a spectral library. The absorbance spectra of the adenosine and AMP samples (pink line in E and F) were the same as the absorbance spectra of the adenosine standard (similarity index = 0.99) and the AMP standard (similarity index = 1.00) (black line in E and F). (G) A significant difference in adenosine signaling molecules was present between the vehicle (open bar, n = 6) and RTX groups (filled bar, n = 5) (ie, decreased adenosine and increased AMP). Elution time scale, minutes. * P < 0.05, ** P < 0.01. Bar, 50 µm.
Nucleodur C18 Gravity Sb Column, supplied by MACHEREY NAGEL, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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macherey nagel nucleodur c18 htec  (MACHEREY NAGEL)
93
MACHEREY NAGEL macherey nagel nucleodur c18 htec
Changes in prostatic acid phosphatase (PAP) ectonucleotidase activity and alterations of adenosine hydrolysis in resiniferatoxin (RTX) neuropathy. (A–C) Prostatic acid phosphatase ectonucleotidase activity was assessed through enzyme histochemistry using adenosine monophosphate (AMP, 3 mM) as a substrate. The reaction product was developed with 1% sodium sulfide and appeared dark brown in the dorsal root ganglion (DRG) sections of the vehicle (A) and day 7 after RTX neuropathy (RTX group) (B). (C) The graph shows AMP(+) neuronal densities in the vehicle (open bar, n = 5) and RTX (filled bar, n = 5) groups according to (A and B). (D–G) High-performance liquid chromatography <t>(HPLC)</t> was conducted to assess the change in the contents of adenosine and AMP, which were extracted from DRG. (D) This graph shows the HPLC chromatograms of the eluted standards (upper panel; AMP, 6.5 minutes; adenosine, 10.2 minutes), the vehicle group (middle panel), and the RTX group (bottom panel). Number designation on the chromatograms: 1 for AMP and 2 for adenosine. (E and F) These figures provide verification of the peaks for AMP (E) and adenosine (F) samples extracted from DRG tissues with respect to a spectral library. The absorbance spectra of the adenosine and AMP samples (pink line in E and F) were the same as the absorbance spectra of the adenosine standard (similarity index = 0.99) and the AMP standard (similarity index = 1.00) (black line in E and F). (G) A significant difference in adenosine signaling molecules was present between the vehicle (open bar, n = 6) and RTX groups (filled bar, n = 5) (ie, decreased adenosine and increased AMP). Elution time scale, minutes. * P < 0.05, ** P < 0.01. Bar, 50 µm.
Macherey Nagel Nucleodur C18 Htec, supplied by MACHEREY NAGEL, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Changes in prostatic acid phosphatase (PAP) ectonucleotidase activity and alterations of adenosine hydrolysis in resiniferatoxin (RTX) neuropathy. (A–C) Prostatic acid phosphatase ectonucleotidase activity was assessed through enzyme histochemistry using adenosine monophosphate (AMP, 3 mM) as a substrate. The reaction product was developed with 1% sodium sulfide and appeared dark brown in the dorsal root ganglion (DRG) sections of the vehicle (A) and day 7 after RTX neuropathy (RTX group) (B). (C) The graph shows AMP(+) neuronal densities in the vehicle (open bar, n = 5) and RTX (filled bar, n = 5) groups according to (A and B). (D–G) High-performance liquid chromatography (HPLC) was conducted to assess the change in the contents of adenosine and AMP, which were extracted from DRG. (D) This graph shows the HPLC chromatograms of the eluted standards (upper panel; AMP, 6.5 minutes; adenosine, 10.2 minutes), the vehicle group (middle panel), and the RTX group (bottom panel). Number designation on the chromatograms: 1 for AMP and 2 for adenosine. (E and F) These figures provide verification of the peaks for AMP (E) and adenosine (F) samples extracted from DRG tissues with respect to a spectral library. The absorbance spectra of the adenosine and AMP samples (pink line in E and F) were the same as the absorbance spectra of the adenosine standard (similarity index = 0.99) and the AMP standard (similarity index = 1.00) (black line in E and F). (G) A significant difference in adenosine signaling molecules was present between the vehicle (open bar, n = 6) and RTX groups (filled bar, n = 5) (ie, decreased adenosine and increased AMP). Elution time scale, minutes. * P < 0.05, ** P < 0.01. Bar, 50 µm.

Journal: Pain

Article Title: Downregulation of adenosine and adenosine A1 receptor contributes to neuropathic pain in resiniferatoxin neuropathy

doi: 10.1097/j.pain.0000000000001246

Figure Lengend Snippet: Changes in prostatic acid phosphatase (PAP) ectonucleotidase activity and alterations of adenosine hydrolysis in resiniferatoxin (RTX) neuropathy. (A–C) Prostatic acid phosphatase ectonucleotidase activity was assessed through enzyme histochemistry using adenosine monophosphate (AMP, 3 mM) as a substrate. The reaction product was developed with 1% sodium sulfide and appeared dark brown in the dorsal root ganglion (DRG) sections of the vehicle (A) and day 7 after RTX neuropathy (RTX group) (B). (C) The graph shows AMP(+) neuronal densities in the vehicle (open bar, n = 5) and RTX (filled bar, n = 5) groups according to (A and B). (D–G) High-performance liquid chromatography (HPLC) was conducted to assess the change in the contents of adenosine and AMP, which were extracted from DRG. (D) This graph shows the HPLC chromatograms of the eluted standards (upper panel; AMP, 6.5 minutes; adenosine, 10.2 minutes), the vehicle group (middle panel), and the RTX group (bottom panel). Number designation on the chromatograms: 1 for AMP and 2 for adenosine. (E and F) These figures provide verification of the peaks for AMP (E) and adenosine (F) samples extracted from DRG tissues with respect to a spectral library. The absorbance spectra of the adenosine and AMP samples (pink line in E and F) were the same as the absorbance spectra of the adenosine standard (similarity index = 0.99) and the AMP standard (similarity index = 1.00) (black line in E and F). (G) A significant difference in adenosine signaling molecules was present between the vehicle (open bar, n = 6) and RTX groups (filled bar, n = 5) (ie, decreased adenosine and increased AMP). Elution time scale, minutes. * P < 0.05, ** P < 0.01. Bar, 50 µm.

Article Snippet: A Brownlee SPP HPLC column (C18, 2.7 μm, 4.6 × 150 mm; PerkinElmer, Waltham, MA) protected with a guard cartilage (C18, 2.7 μm, 4.6 × 5 mm; PerkinElmer) was used in this assay.

Techniques: Activity Assay, High Performance Liquid Chromatography