guard column Search Results


brownlee spp hplc column  (Revvity)
92
Revvity brownlee spp hplc column
Changes in prostatic acid phosphatase (PAP) ectonucleotidase activity and alterations of adenosine hydrolysis in resiniferatoxin (RTX) neuropathy. (A–C) Prostatic acid phosphatase ectonucleotidase activity was assessed through enzyme histochemistry using adenosine monophosphate (AMP, 3 mM) as a substrate. The reaction product was developed with 1% sodium sulfide and appeared dark brown in the dorsal root ganglion (DRG) sections of the vehicle (A) and day 7 after RTX neuropathy (RTX group) (B). (C) The graph shows AMP(+) neuronal densities in the vehicle (open bar, n = 5) and RTX (filled bar, n = 5) groups according to (A and B). (D–G) High-performance liquid chromatography <t>(HPLC)</t> was conducted to assess the change in the contents of adenosine and AMP, which were extracted from DRG. (D) This graph shows the HPLC chromatograms of the eluted standards (upper panel; AMP, 6.5 minutes; adenosine, 10.2 minutes), the vehicle group (middle panel), and the RTX group (bottom panel). Number designation on the chromatograms: 1 for AMP and 2 for adenosine. (E and F) These figures provide verification of the peaks for AMP (E) and adenosine (F) samples extracted from DRG tissues with respect to a spectral library. The absorbance spectra of the adenosine and AMP samples (pink line in E and F) were the same as the absorbance spectra of the adenosine standard (similarity index = 0.99) and the AMP standard (similarity index = 1.00) (black line in E and F). (G) A significant difference in adenosine signaling molecules was present between the vehicle (open bar, n = 6) and RTX groups (filled bar, n = 5) (ie, decreased adenosine and increased AMP). Elution time scale, minutes. * P < 0.05, ** P < 0.01. Bar, 50 µm.
Brownlee Spp Hplc Column, supplied by Revvity, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 92 stars, based on 1 article reviews
brownlee spp hplc column - by Bioz Stars, 2026-03
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15mmopti guard phenyl guard column  (Optimize Technologies)
93
Optimize Technologies 15mmopti guard phenyl guard column
Changes in prostatic acid phosphatase (PAP) ectonucleotidase activity and alterations of adenosine hydrolysis in resiniferatoxin (RTX) neuropathy. (A–C) Prostatic acid phosphatase ectonucleotidase activity was assessed through enzyme histochemistry using adenosine monophosphate (AMP, 3 mM) as a substrate. The reaction product was developed with 1% sodium sulfide and appeared dark brown in the dorsal root ganglion (DRG) sections of the vehicle (A) and day 7 after RTX neuropathy (RTX group) (B). (C) The graph shows AMP(+) neuronal densities in the vehicle (open bar, n = 5) and RTX (filled bar, n = 5) groups according to (A and B). (D–G) High-performance liquid chromatography <t>(HPLC)</t> was conducted to assess the change in the contents of adenosine and AMP, which were extracted from DRG. (D) This graph shows the HPLC chromatograms of the eluted standards (upper panel; AMP, 6.5 minutes; adenosine, 10.2 minutes), the vehicle group (middle panel), and the RTX group (bottom panel). Number designation on the chromatograms: 1 for AMP and 2 for adenosine. (E and F) These figures provide verification of the peaks for AMP (E) and adenosine (F) samples extracted from DRG tissues with respect to a spectral library. The absorbance spectra of the adenosine and AMP samples (pink line in E and F) were the same as the absorbance spectra of the adenosine standard (similarity index = 0.99) and the AMP standard (similarity index = 1.00) (black line in E and F). (G) A significant difference in adenosine signaling molecules was present between the vehicle (open bar, n = 6) and RTX groups (filled bar, n = 5) (ie, decreased adenosine and increased AMP). Elution time scale, minutes. * P < 0.05, ** P < 0.01. Bar, 50 µm.
15mmopti Guard Phenyl Guard Column, supplied by Optimize Technologies, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
15mmopti guard phenyl guard column - by Bioz Stars, 2026-03
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opti lynx zic philic guard column cartridge  (Optimize Technologies)
93
Optimize Technologies opti lynx zic philic guard column cartridge
Changes in prostatic acid phosphatase (PAP) ectonucleotidase activity and alterations of adenosine hydrolysis in resiniferatoxin (RTX) neuropathy. (A–C) Prostatic acid phosphatase ectonucleotidase activity was assessed through enzyme histochemistry using adenosine monophosphate (AMP, 3 mM) as a substrate. The reaction product was developed with 1% sodium sulfide and appeared dark brown in the dorsal root ganglion (DRG) sections of the vehicle (A) and day 7 after RTX neuropathy (RTX group) (B). (C) The graph shows AMP(+) neuronal densities in the vehicle (open bar, n = 5) and RTX (filled bar, n = 5) groups according to (A and B). (D–G) High-performance liquid chromatography <t>(HPLC)</t> was conducted to assess the change in the contents of adenosine and AMP, which were extracted from DRG. (D) This graph shows the HPLC chromatograms of the eluted standards (upper panel; AMP, 6.5 minutes; adenosine, 10.2 minutes), the vehicle group (middle panel), and the RTX group (bottom panel). Number designation on the chromatograms: 1 for AMP and 2 for adenosine. (E and F) These figures provide verification of the peaks for AMP (E) and adenosine (F) samples extracted from DRG tissues with respect to a spectral library. The absorbance spectra of the adenosine and AMP samples (pink line in E and F) were the same as the absorbance spectra of the adenosine standard (similarity index = 0.99) and the AMP standard (similarity index = 1.00) (black line in E and F). (G) A significant difference in adenosine signaling molecules was present between the vehicle (open bar, n = 6) and RTX groups (filled bar, n = 5) (ie, decreased adenosine and increased AMP). Elution time scale, minutes. * P < 0.05, ** P < 0.01. Bar, 50 µm.
Opti Lynx Zic Philic Guard Column Cartridge, supplied by Optimize Technologies, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/opti lynx zic philic guard column cartridge/product/Optimize Technologies
Average 93 stars, based on 1 article reviews
opti lynx zic philic guard column cartridge - by Bioz Stars, 2026-03
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elite 1701  (Revvity)
91
Revvity elite 1701
Changes in prostatic acid phosphatase (PAP) ectonucleotidase activity and alterations of adenosine hydrolysis in resiniferatoxin (RTX) neuropathy. (A–C) Prostatic acid phosphatase ectonucleotidase activity was assessed through enzyme histochemistry using adenosine monophosphate (AMP, 3 mM) as a substrate. The reaction product was developed with 1% sodium sulfide and appeared dark brown in the dorsal root ganglion (DRG) sections of the vehicle (A) and day 7 after RTX neuropathy (RTX group) (B). (C) The graph shows AMP(+) neuronal densities in the vehicle (open bar, n = 5) and RTX (filled bar, n = 5) groups according to (A and B). (D–G) High-performance liquid chromatography <t>(HPLC)</t> was conducted to assess the change in the contents of adenosine and AMP, which were extracted from DRG. (D) This graph shows the HPLC chromatograms of the eluted standards (upper panel; AMP, 6.5 minutes; adenosine, 10.2 minutes), the vehicle group (middle panel), and the RTX group (bottom panel). Number designation on the chromatograms: 1 for AMP and 2 for adenosine. (E and F) These figures provide verification of the peaks for AMP (E) and adenosine (F) samples extracted from DRG tissues with respect to a spectral library. The absorbance spectra of the adenosine and AMP samples (pink line in E and F) were the same as the absorbance spectra of the adenosine standard (similarity index = 0.99) and the AMP standard (similarity index = 1.00) (black line in E and F). (G) A significant difference in adenosine signaling molecules was present between the vehicle (open bar, n = 6) and RTX groups (filled bar, n = 5) (ie, decreased adenosine and increased AMP). Elution time scale, minutes. * P < 0.05, ** P < 0.01. Bar, 50 µm.
Elite 1701, supplied by Revvity, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/elite 1701/product/Revvity
Average 91 stars, based on 1 article reviews
elite 1701 - by Bioz Stars, 2026-03
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spp c 18  (Revvity)
91
Revvity spp c 18
Changes in prostatic acid phosphatase (PAP) ectonucleotidase activity and alterations of adenosine hydrolysis in resiniferatoxin (RTX) neuropathy. (A–C) Prostatic acid phosphatase ectonucleotidase activity was assessed through enzyme histochemistry using adenosine monophosphate (AMP, 3 mM) as a substrate. The reaction product was developed with 1% sodium sulfide and appeared dark brown in the dorsal root ganglion (DRG) sections of the vehicle (A) and day 7 after RTX neuropathy (RTX group) (B). (C) The graph shows AMP(+) neuronal densities in the vehicle (open bar, n = 5) and RTX (filled bar, n = 5) groups according to (A and B). (D–G) High-performance liquid chromatography <t>(HPLC)</t> was conducted to assess the change in the contents of adenosine and AMP, which were extracted from DRG. (D) This graph shows the HPLC chromatograms of the eluted standards (upper panel; AMP, 6.5 minutes; adenosine, 10.2 minutes), the vehicle group (middle panel), and the RTX group (bottom panel). Number designation on the chromatograms: 1 for AMP and 2 for adenosine. (E and F) These figures provide verification of the peaks for AMP (E) and adenosine (F) samples extracted from DRG tissues with respect to a spectral library. The absorbance spectra of the adenosine and AMP samples (pink line in E and F) were the same as the absorbance spectra of the adenosine standard (similarity index = 0.99) and the AMP standard (similarity index = 1.00) (black line in E and F). (G) A significant difference in adenosine signaling molecules was present between the vehicle (open bar, n = 6) and RTX groups (filled bar, n = 5) (ie, decreased adenosine and increased AMP). Elution time scale, minutes. * P < 0.05, ** P < 0.01. Bar, 50 µm.
Spp C 18, supplied by Revvity, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/spp c 18/product/Revvity
Average 91 stars, based on 1 article reviews
spp c 18 - by Bioz Stars, 2026-03
91/100 stars
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cartridge  (Optimize Technologies)
90
Optimize Technologies cartridge
Changes in prostatic acid phosphatase (PAP) ectonucleotidase activity and alterations of adenosine hydrolysis in resiniferatoxin (RTX) neuropathy. (A–C) Prostatic acid phosphatase ectonucleotidase activity was assessed through enzyme histochemistry using adenosine monophosphate (AMP, 3 mM) as a substrate. The reaction product was developed with 1% sodium sulfide and appeared dark brown in the dorsal root ganglion (DRG) sections of the vehicle (A) and day 7 after RTX neuropathy (RTX group) (B). (C) The graph shows AMP(+) neuronal densities in the vehicle (open bar, n = 5) and RTX (filled bar, n = 5) groups according to (A and B). (D–G) High-performance liquid chromatography <t>(HPLC)</t> was conducted to assess the change in the contents of adenosine and AMP, which were extracted from DRG. (D) This graph shows the HPLC chromatograms of the eluted standards (upper panel; AMP, 6.5 minutes; adenosine, 10.2 minutes), the vehicle group (middle panel), and the RTX group (bottom panel). Number designation on the chromatograms: 1 for AMP and 2 for adenosine. (E and F) These figures provide verification of the peaks for AMP (E) and adenosine (F) samples extracted from DRG tissues with respect to a spectral library. The absorbance spectra of the adenosine and AMP samples (pink line in E and F) were the same as the absorbance spectra of the adenosine standard (similarity index = 0.99) and the AMP standard (similarity index = 1.00) (black line in E and F). (G) A significant difference in adenosine signaling molecules was present between the vehicle (open bar, n = 6) and RTX groups (filled bar, n = 5) (ie, decreased adenosine and increased AMP). Elution time scale, minutes. * P < 0.05, ** P < 0.01. Bar, 50 µm.
Cartridge, supplied by Optimize Technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
cartridge - by Bioz Stars, 2026-03
90/100 stars
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hplc apparatus guard column  (Merck KGaA)
90
Merck KGaA hplc apparatus guard column
Changes in prostatic acid phosphatase (PAP) ectonucleotidase activity and alterations of adenosine hydrolysis in resiniferatoxin (RTX) neuropathy. (A–C) Prostatic acid phosphatase ectonucleotidase activity was assessed through enzyme histochemistry using adenosine monophosphate (AMP, 3 mM) as a substrate. The reaction product was developed with 1% sodium sulfide and appeared dark brown in the dorsal root ganglion (DRG) sections of the vehicle (A) and day 7 after RTX neuropathy (RTX group) (B). (C) The graph shows AMP(+) neuronal densities in the vehicle (open bar, n = 5) and RTX (filled bar, n = 5) groups according to (A and B). (D–G) High-performance liquid chromatography <t>(HPLC)</t> was conducted to assess the change in the contents of adenosine and AMP, which were extracted from DRG. (D) This graph shows the HPLC chromatograms of the eluted standards (upper panel; AMP, 6.5 minutes; adenosine, 10.2 minutes), the vehicle group (middle panel), and the RTX group (bottom panel). Number designation on the chromatograms: 1 for AMP and 2 for adenosine. (E and F) These figures provide verification of the peaks for AMP (E) and adenosine (F) samples extracted from DRG tissues with respect to a spectral library. The absorbance spectra of the adenosine and AMP samples (pink line in E and F) were the same as the absorbance spectra of the adenosine standard (similarity index = 0.99) and the AMP standard (similarity index = 1.00) (black line in E and F). (G) A significant difference in adenosine signaling molecules was present between the vehicle (open bar, n = 6) and RTX groups (filled bar, n = 5) (ie, decreased adenosine and increased AMP). Elution time scale, minutes. * P < 0.05, ** P < 0.01. Bar, 50 µm.
Hplc Apparatus Guard Column, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/hplc apparatus guard column/product/Merck KGaA
Average 90 stars, based on 1 article reviews
hplc apparatus guard column - by Bioz Stars, 2026-03
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i-series guard column  (Viscogel Inc)
90
Viscogel Inc i-series guard column
Changes in prostatic acid phosphatase (PAP) ectonucleotidase activity and alterations of adenosine hydrolysis in resiniferatoxin (RTX) neuropathy. (A–C) Prostatic acid phosphatase ectonucleotidase activity was assessed through enzyme histochemistry using adenosine monophosphate (AMP, 3 mM) as a substrate. The reaction product was developed with 1% sodium sulfide and appeared dark brown in the dorsal root ganglion (DRG) sections of the vehicle (A) and day 7 after RTX neuropathy (RTX group) (B). (C) The graph shows AMP(+) neuronal densities in the vehicle (open bar, n = 5) and RTX (filled bar, n = 5) groups according to (A and B). (D–G) High-performance liquid chromatography <t>(HPLC)</t> was conducted to assess the change in the contents of adenosine and AMP, which were extracted from DRG. (D) This graph shows the HPLC chromatograms of the eluted standards (upper panel; AMP, 6.5 minutes; adenosine, 10.2 minutes), the vehicle group (middle panel), and the RTX group (bottom panel). Number designation on the chromatograms: 1 for AMP and 2 for adenosine. (E and F) These figures provide verification of the peaks for AMP (E) and adenosine (F) samples extracted from DRG tissues with respect to a spectral library. The absorbance spectra of the adenosine and AMP samples (pink line in E and F) were the same as the absorbance spectra of the adenosine standard (similarity index = 0.99) and the AMP standard (similarity index = 1.00) (black line in E and F). (G) A significant difference in adenosine signaling molecules was present between the vehicle (open bar, n = 6) and RTX groups (filled bar, n = 5) (ie, decreased adenosine and increased AMP). Elution time scale, minutes. * P < 0.05, ** P < 0.01. Bar, 50 µm.
I Series Guard Column, supplied by Viscogel Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/i-series guard column/product/Viscogel Inc
Average 90 stars, based on 1 article reviews
i-series guard column - by Bioz Stars, 2026-03
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tsk super aw-l guard column  (Tosoh Corporation)
90
Tosoh Corporation tsk super aw-l guard column
Changes in prostatic acid phosphatase (PAP) ectonucleotidase activity and alterations of adenosine hydrolysis in resiniferatoxin (RTX) neuropathy. (A–C) Prostatic acid phosphatase ectonucleotidase activity was assessed through enzyme histochemistry using adenosine monophosphate (AMP, 3 mM) as a substrate. The reaction product was developed with 1% sodium sulfide and appeared dark brown in the dorsal root ganglion (DRG) sections of the vehicle (A) and day 7 after RTX neuropathy (RTX group) (B). (C) The graph shows AMP(+) neuronal densities in the vehicle (open bar, n = 5) and RTX (filled bar, n = 5) groups according to (A and B). (D–G) High-performance liquid chromatography <t>(HPLC)</t> was conducted to assess the change in the contents of adenosine and AMP, which were extracted from DRG. (D) This graph shows the HPLC chromatograms of the eluted standards (upper panel; AMP, 6.5 minutes; adenosine, 10.2 minutes), the vehicle group (middle panel), and the RTX group (bottom panel). Number designation on the chromatograms: 1 for AMP and 2 for adenosine. (E and F) These figures provide verification of the peaks for AMP (E) and adenosine (F) samples extracted from DRG tissues with respect to a spectral library. The absorbance spectra of the adenosine and AMP samples (pink line in E and F) were the same as the absorbance spectra of the adenosine standard (similarity index = 0.99) and the AMP standard (similarity index = 1.00) (black line in E and F). (G) A significant difference in adenosine signaling molecules was present between the vehicle (open bar, n = 6) and RTX groups (filled bar, n = 5) (ie, decreased adenosine and increased AMP). Elution time scale, minutes. * P < 0.05, ** P < 0.01. Bar, 50 µm.
Tsk Super Aw L Guard Column, supplied by Tosoh Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/tsk super aw-l guard column/product/Tosoh Corporation
Average 90 stars, based on 1 article reviews
tsk super aw-l guard column - by Bioz Stars, 2026-03
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chromsep guard column  (Varian Medical)
90
Varian Medical chromsep guard column
Changes in prostatic acid phosphatase (PAP) ectonucleotidase activity and alterations of adenosine hydrolysis in resiniferatoxin (RTX) neuropathy. (A–C) Prostatic acid phosphatase ectonucleotidase activity was assessed through enzyme histochemistry using adenosine monophosphate (AMP, 3 mM) as a substrate. The reaction product was developed with 1% sodium sulfide and appeared dark brown in the dorsal root ganglion (DRG) sections of the vehicle (A) and day 7 after RTX neuropathy (RTX group) (B). (C) The graph shows AMP(+) neuronal densities in the vehicle (open bar, n = 5) and RTX (filled bar, n = 5) groups according to (A and B). (D–G) High-performance liquid chromatography <t>(HPLC)</t> was conducted to assess the change in the contents of adenosine and AMP, which were extracted from DRG. (D) This graph shows the HPLC chromatograms of the eluted standards (upper panel; AMP, 6.5 minutes; adenosine, 10.2 minutes), the vehicle group (middle panel), and the RTX group (bottom panel). Number designation on the chromatograms: 1 for AMP and 2 for adenosine. (E and F) These figures provide verification of the peaks for AMP (E) and adenosine (F) samples extracted from DRG tissues with respect to a spectral library. The absorbance spectra of the adenosine and AMP samples (pink line in E and F) were the same as the absorbance spectra of the adenosine standard (similarity index = 0.99) and the AMP standard (similarity index = 1.00) (black line in E and F). (G) A significant difference in adenosine signaling molecules was present between the vehicle (open bar, n = 6) and RTX groups (filled bar, n = 5) (ie, decreased adenosine and increased AMP). Elution time scale, minutes. * P < 0.05, ** P < 0.01. Bar, 50 µm.
Chromsep Guard Column, supplied by Varian Medical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/chromsep guard column/product/Varian Medical
Average 90 stars, based on 1 article reviews
chromsep guard column - by Bioz Stars, 2026-03
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c18 guard column (7 x)  (Hichrom Limited)
90
Hichrom Limited c18 guard column (7 x)
Changes in prostatic acid phosphatase (PAP) ectonucleotidase activity and alterations of adenosine hydrolysis in resiniferatoxin (RTX) neuropathy. (A–C) Prostatic acid phosphatase ectonucleotidase activity was assessed through enzyme histochemistry using adenosine monophosphate (AMP, 3 mM) as a substrate. The reaction product was developed with 1% sodium sulfide and appeared dark brown in the dorsal root ganglion (DRG) sections of the vehicle (A) and day 7 after RTX neuropathy (RTX group) (B). (C) The graph shows AMP(+) neuronal densities in the vehicle (open bar, n = 5) and RTX (filled bar, n = 5) groups according to (A and B). (D–G) High-performance liquid chromatography <t>(HPLC)</t> was conducted to assess the change in the contents of adenosine and AMP, which were extracted from DRG. (D) This graph shows the HPLC chromatograms of the eluted standards (upper panel; AMP, 6.5 minutes; adenosine, 10.2 minutes), the vehicle group (middle panel), and the RTX group (bottom panel). Number designation on the chromatograms: 1 for AMP and 2 for adenosine. (E and F) These figures provide verification of the peaks for AMP (E) and adenosine (F) samples extracted from DRG tissues with respect to a spectral library. The absorbance spectra of the adenosine and AMP samples (pink line in E and F) were the same as the absorbance spectra of the adenosine standard (similarity index = 0.99) and the AMP standard (similarity index = 1.00) (black line in E and F). (G) A significant difference in adenosine signaling molecules was present between the vehicle (open bar, n = 6) and RTX groups (filled bar, n = 5) (ie, decreased adenosine and increased AMP). Elution time scale, minutes. * P < 0.05, ** P < 0.01. Bar, 50 µm.
C18 Guard Column (7 X), supplied by Hichrom Limited, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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microsorb 100-5 c18  (Dynamax Inc)
90
Dynamax Inc microsorb 100-5 c18
Changes in prostatic acid phosphatase (PAP) ectonucleotidase activity and alterations of adenosine hydrolysis in resiniferatoxin (RTX) neuropathy. (A–C) Prostatic acid phosphatase ectonucleotidase activity was assessed through enzyme histochemistry using adenosine monophosphate (AMP, 3 mM) as a substrate. The reaction product was developed with 1% sodium sulfide and appeared dark brown in the dorsal root ganglion (DRG) sections of the vehicle (A) and day 7 after RTX neuropathy (RTX group) (B). (C) The graph shows AMP(+) neuronal densities in the vehicle (open bar, n = 5) and RTX (filled bar, n = 5) groups according to (A and B). (D–G) High-performance liquid chromatography <t>(HPLC)</t> was conducted to assess the change in the contents of adenosine and AMP, which were extracted from DRG. (D) This graph shows the HPLC chromatograms of the eluted standards (upper panel; AMP, 6.5 minutes; adenosine, 10.2 minutes), the vehicle group (middle panel), and the RTX group (bottom panel). Number designation on the chromatograms: 1 for AMP and 2 for adenosine. (E and F) These figures provide verification of the peaks for AMP (E) and adenosine (F) samples extracted from DRG tissues with respect to a spectral library. The absorbance spectra of the adenosine and AMP samples (pink line in E and F) were the same as the absorbance spectra of the adenosine standard (similarity index = 0.99) and the AMP standard (similarity index = 1.00) (black line in E and F). (G) A significant difference in adenosine signaling molecules was present between the vehicle (open bar, n = 6) and RTX groups (filled bar, n = 5) (ie, decreased adenosine and increased AMP). Elution time scale, minutes. * P < 0.05, ** P < 0.01. Bar, 50 µm.
Microsorb 100 5 C18, supplied by Dynamax Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Changes in prostatic acid phosphatase (PAP) ectonucleotidase activity and alterations of adenosine hydrolysis in resiniferatoxin (RTX) neuropathy. (A–C) Prostatic acid phosphatase ectonucleotidase activity was assessed through enzyme histochemistry using adenosine monophosphate (AMP, 3 mM) as a substrate. The reaction product was developed with 1% sodium sulfide and appeared dark brown in the dorsal root ganglion (DRG) sections of the vehicle (A) and day 7 after RTX neuropathy (RTX group) (B). (C) The graph shows AMP(+) neuronal densities in the vehicle (open bar, n = 5) and RTX (filled bar, n = 5) groups according to (A and B). (D–G) High-performance liquid chromatography (HPLC) was conducted to assess the change in the contents of adenosine and AMP, which were extracted from DRG. (D) This graph shows the HPLC chromatograms of the eluted standards (upper panel; AMP, 6.5 minutes; adenosine, 10.2 minutes), the vehicle group (middle panel), and the RTX group (bottom panel). Number designation on the chromatograms: 1 for AMP and 2 for adenosine. (E and F) These figures provide verification of the peaks for AMP (E) and adenosine (F) samples extracted from DRG tissues with respect to a spectral library. The absorbance spectra of the adenosine and AMP samples (pink line in E and F) were the same as the absorbance spectra of the adenosine standard (similarity index = 0.99) and the AMP standard (similarity index = 1.00) (black line in E and F). (G) A significant difference in adenosine signaling molecules was present between the vehicle (open bar, n = 6) and RTX groups (filled bar, n = 5) (ie, decreased adenosine and increased AMP). Elution time scale, minutes. * P < 0.05, ** P < 0.01. Bar, 50 µm.

Journal: Pain

Article Title: Downregulation of adenosine and adenosine A1 receptor contributes to neuropathic pain in resiniferatoxin neuropathy

doi: 10.1097/j.pain.0000000000001246

Figure Lengend Snippet: Changes in prostatic acid phosphatase (PAP) ectonucleotidase activity and alterations of adenosine hydrolysis in resiniferatoxin (RTX) neuropathy. (A–C) Prostatic acid phosphatase ectonucleotidase activity was assessed through enzyme histochemistry using adenosine monophosphate (AMP, 3 mM) as a substrate. The reaction product was developed with 1% sodium sulfide and appeared dark brown in the dorsal root ganglion (DRG) sections of the vehicle (A) and day 7 after RTX neuropathy (RTX group) (B). (C) The graph shows AMP(+) neuronal densities in the vehicle (open bar, n = 5) and RTX (filled bar, n = 5) groups according to (A and B). (D–G) High-performance liquid chromatography (HPLC) was conducted to assess the change in the contents of adenosine and AMP, which were extracted from DRG. (D) This graph shows the HPLC chromatograms of the eluted standards (upper panel; AMP, 6.5 minutes; adenosine, 10.2 minutes), the vehicle group (middle panel), and the RTX group (bottom panel). Number designation on the chromatograms: 1 for AMP and 2 for adenosine. (E and F) These figures provide verification of the peaks for AMP (E) and adenosine (F) samples extracted from DRG tissues with respect to a spectral library. The absorbance spectra of the adenosine and AMP samples (pink line in E and F) were the same as the absorbance spectra of the adenosine standard (similarity index = 0.99) and the AMP standard (similarity index = 1.00) (black line in E and F). (G) A significant difference in adenosine signaling molecules was present between the vehicle (open bar, n = 6) and RTX groups (filled bar, n = 5) (ie, decreased adenosine and increased AMP). Elution time scale, minutes. * P < 0.05, ** P < 0.01. Bar, 50 µm.

Article Snippet: A Brownlee SPP HPLC column (C18, 2.7 µm, 4.6 × 150 mm; PerkinElmer, Waltham, MA) protected with a guard cartilage (C18, 2.7 µm, 4.6 × 5 mm; PerkinElmer) was used in this assay.

Techniques: Activity Assay, High Performance Liquid Chromatography